DNA was genotyped using the Illumina Addiction biology SNP array (Hodgkinson et al., 2008 (link)) a panel genotyped in the MLS sample using the Illumina GoldenGate platform (Ilumina Inc,. San Diego, CA). Three SNPs were examined in this study (rs279858, rs279826, and rs279827) as they represent a haplotype block associated with alcohol dependence (Edenberg et al., 2004 (link); Villafuerte et al., 2012 (link)), they represent potential functional SNPs (exonic and splice), and they correspond with previous work on GxE interactions (e.g., Dick et al., 2009 (link); Heitzeg et al., 2014 (link)). SNPs rs279858 and rs279826 are in strong LD (r2 > 0.77) with rs279827. Duplicates were included and no discrepancies were observed. All SNPs were in Hardy-Weinberg equilibrium. Findings focus on SNP rs279858 (exon 5, K132K; AA 31.0% [n=93], AG 51.0% [n=153], and GG 18.0% [n = 54]) for simplicity and clarity, although findings were consistent across SNPs and the haplotype block.
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Alcohol Dependence Genetic Associations
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Aa 31
Addiction
Alcohol dependence
Exon
Haplotype
Snps
Corresponding Organization : University of Michigan–Ann Arbor
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Variable analysis
independent variables
- Rs279858 (exon 5, K132K)
- Alcohol dependence
- Hardy-Weinberg equilibrium of SNPs
- Duplicates were included and no discrepancies were observed.
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