Ursolic Acid and Compound US597 Modulate Endothelial Cell Adhesion

Ursolic acid (UA, purity > 90%) was purchased from Xi'an Ocean Biological Engineering Co., China. Compound US597 was synthesized as previously reported [22 (link)]. UA and US597 were dissolved in dimethyl sulfoxide (DMSO) and were used in all experiments. PE-labeled mouse anti-human ICAM-1, VCAM-1, integrin α5, α6 and integrin β1 mAbs, FITC-labeled mouse anti-human integrin α4 mAb, APC-labeled mouse anti-human E-selectin mAb, PE/FITC mouse IgG1 kappa isotype control and APC mouse IgG1 kappa isotype control antibody were all purchased from Becton Dickinson (BD) Pharmingen™. Human interleukin-1 beta (IL-1β) and mouse anti-human beta-actin (β-actin) antibody were purchased from Cell Signaling Technology. Fibronectin, matrigel and endothelial cell growth supplement (ECGS) were obtained from BD Biocoat™. The primary antibodies anti-ICAM-1, anti-integrin α6, anti-integrin β1 anti-E-selectin, anti-FAK, anti-Src, anti-paxillin and anti-PTEN were obtained from Abcam. The secondary antibody goat anti-mouse-IgG horseradish peroxidase was obtained from Promega. All other reagents used in this study were of the highest purity commercially available.

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Xiang L., Chi T., Tang Q., Yang X., Ou M., Chen X., Yu X., Chen J., Ho R.J., Shao J, & Jia L. (2015). A pentacyclic triterpene natural product, ursolic acid and its prodrug US597 inhibit targets within cell adhesion pathway and prevent cancer metastasis. Oncotarget, 6(11), 9295-9312.

Publication 2015

PE/FITC mouse IgG1 kappa isotype control APC mouse IgG1 kappa isotype control antibody Human interleukin-1 beta (IL-1β) mouse anti-human beta-actin (β-actin) antibody Fibronectin matrigel endothelial cell growth supplement (ECGS) anti-ICAM-1 anti-integrin α6 anti-FAK anti-Src anti-paxillin anti-PTEN goat anti-mouse-IgG horseradish peroxidase

Actin Anti igg Antibodies Antibody Beta actin Dimethyl sulfoxide E selectin Endothelial cell Fibronectin Fitc Goat Horseradish peroxidase Human Icam Icam 1 Igg1 Il 1β Integrin Isotype Matrigel Mouse Paxillin Promega Pten Supplement Ursolic acid Vcam 1

Corresponding Organization :

Other organizations : Fuzhou University, Fujian University of Traditional Chinese Medicine, University of Washington

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Variable analysis

independent variables

Ursolic acid (UA, purity > 90%)
Compound US597

dependent variables

ICAM-1 expression
VCAM-1 expression
Integrin α5 expression
Integrin α6 expression
Integrin β1 expression
Integrin α4 expression
E-selectin expression
FAK expression
Src expression
Paxillin expression
PTEN expression

control variables

Dimethyl sulfoxide (DMSO) used to dissolve UA and US597
PE-labeled mouse anti-human ICAM-1, VCAM-1, integrin α5, α6 and integrin β1 mAbs
FITC-labeled mouse anti-human integrin α4 mAb
APC-labeled mouse anti-human E-selectin mAb
PE/FITC mouse IgG1 kappa isotype control and APC mouse IgG1 kappa isotype control antibody
Human interleukin-1 beta (IL-1β)
Mouse anti-human beta-actin (β-actin) antibody
Fibronectin, matrigel and endothelial cell growth supplement (ECGS)
Anti-ICAM-1, anti-integrin α6, anti-integrin β1, anti-E-selectin, anti-FAK, anti-Src, anti-paxillin and anti-PTEN primary antibodies
Goat anti-mouse-IgG horseradish peroxidase secondary antibody

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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