miRNA microarray analysis was performed as previously described (30 (link),61 (link)). Total RNA from EA.hy926 cells either Mock-infected or infected with K1 or Nef lentivirus alone, or co-infected with both K1 and Nef lentiviruses for 72 h were isolated with Trizol reagent (Invitrogen). The miRNA fraction was further purified using a mirVanaTM miRNA isolation kit (Ambion, Austin, TX, USA). The isolated miRNAs from the four parallel infected cells were then labeled with Hy3 using the miRCURYTM Array Labelling Kit (Exiqon, Vedbaek, Denmark) and hybridized individually on miRCURYTM LNA microRNA Arrays (v 8.0; Exiqon). Microarray images were acquired using a Genepix 4000B scanner (Axon Instruments, Union City, CA, USA), processed and analyzed with Genepix Pro 6.0 software (Axon Instruments). EA.hy926 cells transduced with Mock were used as the control.
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miRNA Microarray Analysis of Lentiviral Infection
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Corresponding Organization :
Other organizations : Nanjing Medical University, Xuzhou Medical College, Quzhou College of Technology, Jiangsu Province Hospital, UPMC Hillman Cancer Center, University of Southern California
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Variable analysis
independent variables
- Infection status: Mock-infected, infected with K1 lentivirus alone, infected with Nef lentivirus alone, or co-infected with both K1 and Nef lentiviruses
- MiRNA expression profile in EA.hy926 cells
- EA.hy926 cells transduced with Mock were used as the control
- Positive control: Not specified
- Negative control: EA.hy926 cells transduced with Mock
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