Doxorubicin Delivery via Magnetic Nanoparticles

Bovine serum albumin, 3-(4,5-dimethyl-2-thiazyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT), dimethyl sulfoxide, phosphate-buffered saline (pH 7.4), N-dicyclohex-ylcarbodiimide, dicyclohexylurea, N-hydroxysuccinimide (NHS), and doxorubicin hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Ammonium ferric sulfate dodecahydrate, hydroxylamine hydrochloride, 1,10-phenanthroline, and sodium acetate were purchased from JiangLai Biological (Shanghai, People’s Republic of China). All solvents were of high-performance liquid chromatography grade. An Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit was supplied by Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). Fe₃O₄ was prepared as described by Li et al.22 (link) All reagents were of analytical grade.

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Yang R., An Y., Miao F., Li M., Liu P, & Tang Q. (2014). Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo. International Journal of Nanomedicine, 9, 4231-4243.

Publication 2014

Bovine serum albumin phosphate-buffered saline (pH 7.4), N-dicyclohex-ylcarbodiimide N-hydroxysuccinimide (NHS), doxorubicin hydrochloride Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit

Ammonium ferric sulfate dodecahydrate Annexin v Apoptosis Biological Bovine serum albumin Bromide Dimethyl sulfoxide Diphenyl Doxorubicin hydrochloride Fe3o4 Fluorescein High performance liquid chromatography Hydroxylamine hydrochloride Isothiocyanate N hydroxysuccinimide Phenanthroline Phosphate Saline Sodium acetate Solvents Tetrazolium

Corresponding Organization :

Other organizations : Southeast University, Zhongda Hospital Southeast University

Top 5 similar protocols

Variable analysis

independent variables

Bovine serum albumin
3-(4,5-dimethyl-2-thiazyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT)
Doxorubicin hydrochloride
Fe3O4 (as described by Li et al.)

dependent variables

Cell viability/cytotoxicity (as measured by MTT assay)

control variables

Phosphate-buffered saline (pH 7.4)
N-dicyclohex-ylcarbodiimide
Dicyclohexylurea
N-hydroxysuccinimide (NHS)
Ammonium ferric sulfate dodecahydrate
Hydroxylamine hydrochloride
1,10-phenanthroline
Sodium acetate
Solvents (high-performance liquid chromatography grade)
Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit

Annotations

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