CD29, CD90, CD105, and CD34 MSC markers were quantified using flow cytometric analysis. Florescent Activated cell sorting (FACS) analysis was used. After brief centrifugation, cells were resuspended in a wash buffer (BD Biosciences, Germany). A total of 300 ml of cell suspension was incubated with antibodies against CD29, CD105, CD34, and CD90 conjugated with Allophycocyanin (APC), Cyanine 5 (CY5), Phycoerythrin (PE), and Fluorescein isothiocyanate (FITC) dyes for 45 min at room temperature. Flow cytometry was performed on a FACS Calibur (BD Biosciences, Germany), and Cell Quest software was used for analysis. Cells are identified by flow cytometry, which was positive for CD29, CD90&CD105, and negative for CD34 (Figure 1 ).
MSCs cells were harvested and labeled with PKH26 fluorescent linker dye (Elberry et al., 2016 (link)).
The remaining thirty animals were divided into five groups (six rats/group):
Cognitive and behavioral tests were performed twice (at the start of the work and the end of the work before euthanasia during the light phase of the light–dark cycles). Blood samples were collected (from orbital sinuses) to measure the serum fasting blood glucose level and insulin level. After the euthanasia, the brains were extracted for biochemical and histological evaluation of the hippocampus.
MSCs cells were harvested and labeled with PKH26 fluorescent linker dye (Elberry et al., 2016 (link)).
The remaining thirty animals were divided into five groups (six rats/group):
Cognitive and behavioral tests were performed twice (at the start of the work and the end of the work before euthanasia during the light phase of the light–dark cycles). Blood samples were collected (from orbital sinuses) to measure the serum fasting blood glucose level and insulin level. After the euthanasia, the brains were extracted for biochemical and histological evaluation of the hippocampus.
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