To measure serum ENL and END concentrations, samples underwent solid-phase extraction and overnight enzymatic hydrolysis. The unconjugated lignans were then isolated by solid-phase extraction, converted to tert-butyldimethylsilyl ethers, and analyzed by gas chromatography mass spectrometry [28 (link)]. Serum cytokines were analyzed by Bio-Plex Multiplex Immunoassay on a Bio-Plex Magpix Multiplex Reader (Bio-Rad, Inc., Hercules, CA, USA).
Free full text: Click here