Evaluating Antioxidant and Energy Dynamics

The Caspase 9 Activity Assay Kit (Beyotime, China, Cat. No: C1158) was used to measure the activity of caspase-9 according to the manufacturer’s instructions [23 (link)]. The concentrations of GSH, SOD and GPX were evaluated using commercial kits (Cellular Glutathione Peroxidase Assay Kit, Beyotime, China, Cat. No: S0056; Glutathione Reductase Assay Kit, Beyotime, China, Cat. No: S0055; Total Superoxide Dismutase Assay Kit, Beyotime, China, Cat. No: S0101, respectively) [24 (link)]. ATP production was measured using a luciferase-based ATP assay kit (Beyotime Institute of Biotechnology) with a microplate reader [25 (link)].

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Yao W., Zhu S., Li P, & Zhang S. (2019). Large tumor suppressor kinase 2 overexpression attenuates 5-FU-resistance in colorectal cancer via activating the JNK-MIEF1-mitochondrial division pathway. Cancer Cell International, 19, 97.

Publication 2019

Cellular Glutathione Peroxidase Assay Kit Glutathione Reductase Assay Kit Total Superoxide Dismutase Assay Kit luciferase-based ATP assay kit

Assay Caspase 9 Cellular Glutathione peroxidase Glutathione reductase Luciferase Superoxide dismutase

Corresponding Organization :

Other organizations : National Clinical Research Center for Digestive Diseases, Capital Medical University, Beijing Friendship Hospital

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Variable analysis

dependent variables

Caspase-9 activity
ATP production

control variables

Not explicitly mentioned

controls

Caspase-9 Activity Assay Kit (Beyotime, China, Cat. No: C1158)
Cellular Glutathione Peroxidase Assay Kit (Beyotime, China, Cat. No: S0056), Glutathione Reductase Assay Kit (Beyotime, China, Cat. No: S0055), Total Superoxide Dismutase Assay Kit (Beyotime, China, Cat. No: S0101), ATP assay kit (Beyotime Institute of Biotechnology)

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