Quantification of Glucose Content in SCF

SBC was determined following a method modified from Dubey et al. [20 (link)]. First, 0.1 g of SCF was dispersed in 2.5 mL deionized water. Subsequently, 5 mL 0.01 M glucose was added, and the mixture was subjected to shaking under incubated conditions at 200 rpm and 37 °C for 6 h (SB-12l, Benchmark Scientific, Sayreville, NJ, USA). The mixture was centrifuged at 2000× g for 15 min, and the supernatant was filtered through a 0.45 µm filter. Then, 2 mL of filtrate was collected and subsequently analyzed using the phenol-sulfuric assay. Absorbance of the mixtures was recorded at 490 nm (Cary 60 UV-Vis Spectrophotometer, Agilent Technologies, Santa Clara, CA, USA) [20 (link)]. To quantify the glucose content of SCF, absorbance values of the samples were compared against a glucose standard curve.