WT and their littermate mgR mice at 7 days old were anesthetized and underwent thoracotomy. Mouse thoracic aortas were isolated and minced. SMC isolation was described previously [22 (link)]. The cells were grown to confluence and passed after trypsinization with 0.25% trypsin. Mouse SMC were maintained using the Vascular Smooth Muscle Cell Growth Kit (ATCC). Experiments were performed on cells from passage 2–4.
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