Facial Modeling and Wrinkle Analysis

The BTBP 3D Clarity Pro^® Facial Modeling and Analysis System (Brigh-Tex BioPhotonics, San Jose, CA, USA) was utilized to obtain high-resolution facial photographs for all study participants at baseline and at 8, 16, and 24 weeks. Facial wrinkles were analyzed through the BTBP 3D Clarity Pro^® Facial Modeling and Analysis System (Brigh-Tex BioPhotonics), which uses an algorithm-based model to measure and calculate the average wrinkle severity based on the depth and width [43 (link)]. Average piment intensity score across the face was also measured utilizing the same image analysis system. The following skin biophysical measures were assessed: hydration (MoistureMeterSC; Delfin Technologies, Stamford, CT, USA) and sebum production in µg/cm² (Sebumeter^® SM 815; Courage and Khazaka, Cologne, Germany). Skin hydration (MoistureMeterSC; Delfin Technologies) and sebum excretion (Sebumeter^® SM 815; Courage and Khazaka) were measured utilizing handheld non-invasive devices at the left and right buccal areas at 0, 8, 16, and 24 weeks. All subjects were acclimated to ambient temperature for fifteen minutes in a temperature-controlled room prior to any skin biophysical measurements. Subjects were instructed to refrain from applying topical products prior to study visits.