Immunofluorescence Staining of Mouse Synaptic Proteins

We used the following mouse monoclonal antibodies: anti-Bassoon (Enzo, Life Sciences cat. no.: O88778); anti-Synapsin, anti-Synaptophysin, anti-Synaptotagmin-1 (Synaptic Systems cat. no.: 106001, 101011, 104202); anti-TGN38 (BD Biosciences cat. no.: 610899). In addition we used the following rabbit polyclonal antibodies: anti-GFP 6556 (Abcam cat. no.: ab6556); anti-MAP2, anti-Mover, anti-TRIM 36, anti-VAMP2, (Synaptic Systems cat. no.: 188004, 248003, 377003, 104203); anti-Rogdi, directed against amino acids 14–95 of human Rogdi (Sigma-Aldrich cat. no.: hpa041000); anti-Rogdi, directed against full-length human Rogdi GST-fusion protein (Proteintech cat. No.: 17047–1-AP), validated by knockdown^{10 (link)}. In addition, we used the following guinea pig polyclonal antibodies: anti-MAP2, anti-Neurofilament H (Synaptic Systems cat. no.: 188004, 171104).