Uninfected and infected HKM (1 × 106) were stained with AV-FITC-PI as described earlier and following manufacturer’s instructions (BD Pharmingen)15 (link) The cells were observed under fluorescence microscope (Nikon Eclipse 400) within 30 min of adding the dye. Three fields with 100 cells each were studied to determine the percentage of apoptotic HKM15 (link).
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