The pancreas and lungs were fixed in 10% neutral buffered formalin, embedded in paraffin, cut into 4 µm-thick sections, and stained with hematoxylin and eosin (H&E) for
histopathological examination. Images were captured with a VS120 Virtual Slide Scanner (Olympus Corp., Tokyo, Japan). The grading of pancreatitis was evaluated using a system previously
described for L-Arg-induced pancreatitis [19 (link)]. The histopathological lesions were scored by an extensively certified veterinary pathologist (T.I.) as
follows: inflammatory cell infiltration (0, none; 1, mild; 2, moderate; 3, severe), acinar cell necrosis (0, none; 1, mild; 2, moderate; 3, severe), and interstitial edema (0, none; 1, mild;
2, moderate; 3, severe) [19 (link)]. Histopathological evaluation of L-Arg-related lung injury was assessed based on alveolar wall thickness, as previously
described [19 (link)]. Alveolar wall thickness was measured using the ImageJ software (Wayne Rasband, NIH). The severity of lung injury was assessed by the
alveolar area calculated from alveolar wall thickness in H&E-stained lung sections (×200) using a previously described method [21 (link)] with some
modifications.
histopathological examination. Images were captured with a VS120 Virtual Slide Scanner (Olympus Corp., Tokyo, Japan). The grading of pancreatitis was evaluated using a system previously
described for L-Arg-induced pancreatitis [19 (link)]. The histopathological lesions were scored by an extensively certified veterinary pathologist (T.I.) as
follows: inflammatory cell infiltration (0, none; 1, mild; 2, moderate; 3, severe), acinar cell necrosis (0, none; 1, mild; 2, moderate; 3, severe), and interstitial edema (0, none; 1, mild;
2, moderate; 3, severe) [19 (link)]. Histopathological evaluation of L-Arg-related lung injury was assessed based on alveolar wall thickness, as previously
described [19 (link)]. Alveolar wall thickness was measured using the ImageJ software (Wayne Rasband, NIH). The severity of lung injury was assessed by the
alveolar area calculated from alveolar wall thickness in H&E-stained lung sections (×200) using a previously described method [21 (link)] with some
modifications.
