After the administration of the last doses of FC or CN,
the brain homogenates were prepared at 1, 2, 4, 8, 16, 24,
48, and 72 hour(s) post-treatment to measure the brain
concentrations of the FC and CN in the rats. According to
the method of Shinde and Devarajan (18 (link)), curcumin was
extracted from the brain homogenates using the coupled
high-performance liquid chromatography (HPLC) with
2487 Dual Absorbance Detector and Waters 515 Pump
Reversed Phase C18 Column. The peaks were read at
425 nm.
Interestingly, HPLC has become a routine tool for the
separation of complex mixtures. However, the ability to
obtain structural information on substances separated
using HPLC is limited by the online detector systems
and, in most applications, full structural elucidation is
performed off-line following separation.
the brain homogenates were prepared at 1, 2, 4, 8, 16, 24,
48, and 72 hour(s) post-treatment to measure the brain
concentrations of the FC and CN in the rats. According to
the method of Shinde and Devarajan (18 (link)), curcumin was
extracted from the brain homogenates using the coupled
high-performance liquid chromatography (HPLC) with
2487 Dual Absorbance Detector and Waters 515 Pump
Reversed Phase C18 Column. The peaks were read at
425 nm.
Interestingly, HPLC has become a routine tool for the
separation of complex mixtures. However, the ability to
obtain structural information on substances separated
using HPLC is limited by the online detector systems
and, in most applications, full structural elucidation is
performed off-line following separation.
