Hippocampi from male mice were collected as previously described (Gjoneska et al., 2015 (link)) immediately after euthanasia. ChIP was then performed as described in Gjoneska et al. (2015) (link). In brief, tissues were minced and crosslinked in 1% formaldehyde (28906; Thermo Fisher Scientific) for 15 min at room temperature and quenched with glycine for 5 min (g7126; Sigma-Aldrich). The samples were homogenized in cell lysis buffer containing proteinase inhibitors (C762Q77; Thermo Fisher Scientific) and chromatin was then fragmented to a size range of 200–500 bp using a digital sonifier (SFX 250; Branson). Solubilized chromatin was then diluted and incubated with 1 µg antibody (sc-352x; Santa Cruz) at 4°C overnight. Immune complexes were captured with Protein A sepharose beads (101041; Thermo Fisher Scientific), washed, and eluted. Enriched chromatin was then subjected to crosslink reversal and proteinase K (25530049; Thermo Fisher Scientific) digestion at 65°C, phenol-chloroform (17908; Thermo Fisher Scientific) extraction, and ethanol precipitation.