The potassium ferricyanide trichloroacetic acid method described by Benzie and Strain (1996) (link) was used to examine the effect of solvent polarity on the total reducing capability of the EPS. The measurements were carried out with a microtiter plate reader (Biotek ELX800; Biotek, Winooski, VT, United States) at a wavelength of 630 nm. Ascorbic acid at a 1 mg/mL dosage was utilized as the positive control in the experiment, while DMSO acted as the negative control. The results were measured and reported as ascorbic acid equivalent (AAE) μg/mg of EPS.
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