Gene expression profiles were downloaded from the GEO database (http://www.ncbi.nlm.nih.gov/geo ). GSE19187 includes 14 cases of isolated AR without asthma and 11 healthy control samples. The expression profiling of nasal epithelial cells collected by brushing had been performed on the GPL6244 platform (Affymetrix Human Genome 1.0 ST Array);18 (link)
GSE44037 includes five cases of AR and six healthy control samples, based on the GPL13158 platform (Affymetrix HT HG-U133+ PM Array);19 (link)
and GSE43523 includes seven cases of SAR and five healthy control samples, based on the GPL6883 platform (Illumina HumanRef-8 v3.0 expression beadchip). Datasets GSE44037 and GSE43523 were merged as validation data to validate the critical genes. The expression levels of CD44, HLA-DPA1, HLA-DRB1, HLA-DRB5, MUC5B, and CD274 were compared between AR and healthy control samples using the Wilcoxon test. The subject characteristics of the dataset are listed in Supplementary Table 1 and the genes detected by microarray data are listed in Supplementary Table 2. All data are freely available online, and this study did not involve any experiments on humans or animals performed by any of the authors. Ethical approval was not required because this study used publicly available datasets.
GSE44037 includes five cases of AR and six healthy control samples, based on the GPL13158 platform (Affymetrix HT HG-U133+ PM Array);19 (link)
and GSE43523 includes seven cases of SAR and five healthy control samples, based on the GPL6883 platform (Illumina HumanRef-8 v3.0 expression beadchip). Datasets GSE44037 and GSE43523 were merged as validation data to validate the critical genes. The expression levels of CD44, HLA-DPA1, HLA-DRB1, HLA-DRB5, MUC5B, and CD274 were compared between AR and healthy control samples using the Wilcoxon test. The subject characteristics of the dataset are listed in Supplementary Table 1 and the genes detected by microarray data are listed in Supplementary Table 2. All data are freely available online, and this study did not involve any experiments on humans or animals performed by any of the authors. Ethical approval was not required because this study used publicly available datasets.
