All mouse procedures were conducted in accordance with the Fred Hutchinson Cancer Center (FHCC) Institutional Animal Care and Use Committee (IACUC) guidelines and the ARRIVE guidelines. C57BL/6J (BL/6J) mice (000664, JAX) were purchased from the Jackson Laboratory. Injections of organoid cultures for the generation of subcutaneously grafted organoid tumors were conducted as described previously (47 (link)). Typically, 1 × 106 to 2 × 106 cells in a 100-μl suspension of 50% Matrigel (Corning) in phosphate-buffered saline (PBS) were injected into each flank of the mouse. When average tumor diameters of 3 to 5 mm3 were reached, mice were treated with a once daily intraperitoneal injection of vehicle or flavopiridol (5 mg/kg; S1230, Selleck) diluted with 5% dextrose in water solution (600063, Bound Tree Medical) for 21 days. Caliper measurements of tumors and body weights were recorded three times per week.
Protocol detail
Xenograft Mouse Model of Organoid Tumors
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Body weights
Cancer
Cells
Dextrose
Flavopiridol
Institutional animal care and use committee
Intraperitoneal injection
Matrigel
Mice
Organoid
Phosphate
Saline
Tree
Tumors
Corresponding Organization : University of Washington
Other organizations : University of Iowa, Princess Margaret Cancer Centre, University Health Network, Friedrich-Alexander-Universität Erlangen-Nürnberg, University of Pennsylvania, University of Toronto, University of Washington Medical Center
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Variable analysis
independent variables
- Flavopiridol (5 mg/kg)
- Tumor diameter (mm^3)
- Body weight
- Mouse strain (C57BL/6J)
- Injection protocol (1 × 10^6 to 2 × 10^6 cells in 100-μl suspension of 50% Matrigel in PBS)
- Injection site (subcutaneous on each flank)
- Treatment protocol (once daily intraperitoneal injection for 21 days)
- Positive control: Vehicle (5% dextrose in water solution)
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