MERS-CoV
pseudovirus was generated via cotransfection of 293T cells with the
plasmid pNL4-3.luc.RE as well as the pcDNA3.1-MERS-CoV-S plasmid,
as previously described.42 (link) Peptides at
graded concentrations were mixed with the pseudovirus and then incubated
for 1 h at room temperature. The mixture was added to Huh-7 or Calu-3
cells, fresh medium was added 12 h later, and the cells were incubated
for an additional 48 h at 37 °C. Fluorescence was determined
immediately using a luciferase kit (Promega) and an Ultra 384 luminometer
(Tecan) after the addition of luciferase substrate (Promega).
pseudovirus was generated via cotransfection of 293T cells with the
plasmid pNL4-3.luc.RE as well as the pcDNA3.1-MERS-CoV-S plasmid,
as previously described.42 (link) Peptides at
graded concentrations were mixed with the pseudovirus and then incubated
for 1 h at room temperature. The mixture was added to Huh-7 or Calu-3
cells, fresh medium was added 12 h later, and the cells were incubated
for an additional 48 h at 37 °C. Fluorescence was determined
immediately using a luciferase kit (Promega) and an Ultra 384 luminometer
(Tecan) after the addition of luciferase substrate (Promega).
