Kidney sections and immunohistochemical staining were performed as previously described (Liu et al., 2020 (link)). Primary antibodies included rabbit polyclonal anti–Wnt1 (ab15251; Abcam, Inc.), rabbit polyclonal anti-β-catenin (ab15180; Abcam, Inc.), and rabbit polyclonal anti-fibronectin (F3648; Sigma-Aldrich).
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