The cultures were harvested after 3 days upon reaching the end of the logarithmic phase. Before harvesting, each HB was sampled for dry cell weight (DCW) measurements and immunoblotting (biological triplicates). The pellets for DCW were obtained from 50 mL samples after centrifugation (5000 g, 10 min, 12 °C) and stored at –20 °C for future freeze drying. For immunoblotting, 10 mL samples were centrifuged as previously described, snap-frozen in liquid nitrogen and stored at –80 °C for later analyses.
Pilot-scale Microalgae Cultivation in Hanging-bag Bioreactors
The cultures were harvested after 3 days upon reaching the end of the logarithmic phase. Before harvesting, each HB was sampled for dry cell weight (DCW) measurements and immunoblotting (biological triplicates). The pellets for DCW were obtained from 50 mL samples after centrifugation (5000 g, 10 min, 12 °C) and stored at –20 °C for future freeze drying. For immunoblotting, 10 mL samples were centrifuged as previously described, snap-frozen in liquid nitrogen and stored at –80 °C for later analyses.
Corresponding Organization : University College London
Other organizations : Institute of Structural and Molecular Biology
Variable analysis
- Illumination provided by three light-emitting diode (LED) panels with an average light intensity of 140 µmol.m^-2.s^-1
- Optical density (OD) at 750 nm
- Dry cell weight (DCW)
- Immunoblotting
- Temperature-controlled room at 24.5 °C
- Constant air flowrate of 1 L/min
- No carbon dioxide supplementation
- Fresh TAP medium as blank for OD measurements
- A late logarithmic-phase culture was used to inoculate the hanging-bag photobioreactors to an initial optical density (OD) reading of 0.015 at 750 nm
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