High-Dose Chemotherapy Conditioning for ASCT

Prior to ASCT on day 0, patients received high-dose chemotherapy with a combination of thiotepa (250 mg/m² per day, from day 9 to day 7), busulfan (3.2 mg/kg per day, from day 6 to day 5, and 1.6 mg/kg on day 4), and cyclophosphamide (60 mg/kg per day, on days 3 and 2). For patients aged 60 and over, the busulfan dose was reduced by 40%. To prevent seizures, clonazepam (0.5 mg/12 h) was administered during busulfan treatment. To prevent hemorrhagic cystitis, mesna was continuously infused during the cyclophosphamide perfusion. Patients received prophylaxis for pneumocystis and viral infections but not for fungal infections. Depending on each center’s practices, some patients received granulocyte colony-stimulating factor during the ASCT.