Rac1 Inhibitor Effects on Lamprey Epidermis

Lampreys treated with the Rac1 inhibitor were cultured using the same above-described approach. The working solution was prepared using DMSO, PEG300 and Tween-80 according to the manufacturer’s instructions (Shanghai Yuanye Biological, Shanghai, China). Subsequently, 1.8 mL of physiological salt solution was added, and the samples were stored at −20 °C. The above-described method was used to damage the epidermis of the lampreys, and before damage, the lampreys were injected with the inhibitor before damage at a low (15 mg/kg) or high concentration (50 mg/kg) or with the carrier control (DMSO, PEG300, Tween-80); the inhibitor was injected subcutaneously every other day. The damaged tissues were collected after 2 and 7 days for staining.