In Vivo Lentivirus Delivery to Rete Testis

Cynomolgus macaques were anesthetized with a 10 mg/kg ketamine chloride intramuscular injection. The rete showed a linear echo dense structure under ultrasound (GE Healthcare, LOGIQ E10). The EGFP lentivirus diluent was loaded into a syringe with a needle (KINDLY GROUP, Shanghai, 0.7 × 80 TWLB, www.kdlchina.cn, accessed on 5 June 2021.)) and then injected into the rete of both testicles under the guidance of an ultrasound system. The needle bevel was punctured into the rete of testicular tissue and EGFP lentivirus vehicles were injected from the epididymis head into the rete [29 (link)].

Free full text: Click here

Wang S., Duan Y., Chen B., Qiu S., Huang T, & Si W. (2023). Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys. Veterinary Sciences, 10(2), 104.

Publication 2023

LOGIQ E10

Chloride Cynomolgus macaques Echo Epididymis Head Intramuscular injection Ketamine Lentivirus Needle Rete testicular Syringe Testicles Tissue Ultrasound

Corresponding Organization : Yunnan Yingmao Biotechnology (China)

Top 5 similar protocols

Variable analysis

independent variables

Injection of EGFP lentivirus diluent into the rete of both testicles under the guidance of an ultrasound system

dependent variables

Observation of the rete structure using ultrasound (GE Healthcare, LOGIQ E10)

control variables

Anesthesia with 10 mg/kg ketamine chloride intramuscular injection
Use of a needle (KINDLY GROUP, Shanghai, 0.7 × 80 TWLB) to inject the EGFP lentivirus diluent

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!