The morphology of strain NIT-SL11 was observed under a field-emission scanning electron microscope (JSM-7800F; JEOL Ltd., Tokyo, Japan) operating at 1.0 kV [19 (link)], and its spore-forming ability and Gram-stainability were checked using optical microscopy, as previously described [24 (link)]. The salinity, temperature, and pH tolerance of NIT-SL11 were evaluated by measuring the growth of cells in DHB-CO3-AF medium. Salinity tolerance was examined by supplementing with 0 to 8% (w/v) of NaCl. The pH of the bicarbonate-free medium was adjusted to the range of 5.2 to 8.6 by the addition of sodium bicarbonate in the medium and CO2 in the headspace for the pH tolerance tests. The cell growth at different temperatures was tested in the range between 4 °C and 10 to 40 °C, with approximately 5 °C intervals.
The ability of strain NIT-SL11 to utilize an e− donor was determined by observing cell growth with the following substances in combination with reduction of 5 mM fumarate: 10 mM of formate, acetate, butyrate, lactate, pyruvate, succinate, propionate, malate, isobutyrate, caproate, benzoate, phenol, methanol, isopropanol, ethanol, butanol, glucose, fructose, and glycerol, and 0.5 g/L of peptone and yeast extract. Potential electron acceptors used by strain NIT-SL11 were assayed by observing cell growth in 10 mM each of fumarate, malate, sulfate, and thiosulfate; 5 mM of AQDS and nitrate; and 20 g/L of elemental sulfur with oxidation of 5 mM acetate. The production of electric current by the strain NIT-SL11 was evaluated via electrochemical cultivation using a graphite plate inoculated with NIT-SL11, as previously described [12 (link)].
The ability of strain NIT-SL11 to utilize an e− donor was determined by observing cell growth with the following substances in combination with reduction of 5 mM fumarate: 10 mM of formate, acetate, butyrate, lactate, pyruvate, succinate, propionate, malate, isobutyrate, caproate, benzoate, phenol, methanol, isopropanol, ethanol, butanol, glucose, fructose, and glycerol, and 0.5 g/L of peptone and yeast extract. Potential electron acceptors used by strain NIT-SL11 were assayed by observing cell growth in 10 mM each of fumarate, malate, sulfate, and thiosulfate; 5 mM of AQDS and nitrate; and 20 g/L of elemental sulfur with oxidation of 5 mM acetate. The production of electric current by the strain NIT-SL11 was evaluated via electrochemical cultivation using a graphite plate inoculated with NIT-SL11, as previously described [12 (link)].
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