4-PBA Intervention for JEV Infection

JEV (10^6.5 TCID₅₀) was intracranially injected into the 5-week-old BALB/c mice. The 4-PBA diluent is recommended by the instructions. 4-PBA (80 mg/kg) is injected once before the challenge. After the challenge, 4-PBA is injected once a day for a total of 9 times. After the blood is collected from the eye vein of mice, the brain tissue is taken for pathological section, immunohistochemistry, Western blot and qPCR. Pathological section and immunohistochemistry experiment and result analysis are contracted by Wuhan Pinofei Biotechnology Co., Ltd., Wuhan, China.

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Wang S., Yang K., Li C., Liu W., Gao T., Yuan F., Guo R., Liu Z., Tan Y., Hu X., Tian Y, & Zhou D. (2023). 4-Phenyl-butyric Acid Inhibits Japanese Encephalitis Virus Replication via Inhibiting Endoplasmic Reticulum Stress Response. Viruses, 15(2), 534.

Publication 2023

4 pba Balb c mice Blood Brain Immunohistochemistry Mice Tissue Vein Western blot

Corresponding Organization : Ministry of Agriculture and Rural Affairs

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Variable analysis

independent variables

Injection of 4-PBA (80 mg/kg) once before the challenge
Injection of 4-PBA once a day for a total of 9 times after the challenge

dependent variables

Pathological changes in the brain tissue
Immunohistochemistry results
Western blot analysis
QPCR analysis

control variables

5-week-old BALB/c mice
4-PBA diluent recommended by the instructions

controls

Positive control: None specified
Negative control: None specified

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