200μg of α-CD8 (clone 2.43, BioXCell), α-CD4 (clone GK1.5, BioXCell), α-PD-1 (clone RMP1–14, BioXCell), or respective isotype controls were administered to mice via intraperitoneal injection on days 5, 7, 9, and 11 post-initial tumor challenge. NK cell depletion experiments followed the same dosing protocol, using 250μg of α-NK1.1 (clone PK136, BioXCell). CLEC9A blocking experiments followed the same dosing protocol, using 400μg of α-CLEC9A (clone 7H11, BioXCell) or isotype control, as described (28 (link)).
Protocol detail
Modulating Antitumor Immunity via Checkpoint Inhibitors
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Clec9a
Clone
Intraperitoneal injection
Isotype
Mice
Nk cell
Tumor
Corresponding Organization : University of Washington
Other organizations : Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- α-CD8 (clone 2.43, BioXCell)
- α-CD4 (clone GK1.5, BioXCell)
- α-PD-1 (clone RMP1–14, BioXCell)
- α-NK1.1 (clone PK136, BioXCell)
- α-CLEC9A (clone 7H11, BioXCell)
- Tumor growth
- Respective isotype controls
- Positive control: Respective isotype controls
- Negative control: Not explicitly mentioned
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