Three intervals of retinal vascular development were evaluated: early (P16 to P20), mid (P23 to P27), late (P30 to P34), and mature (P47) phases. The mature phase denotes any time point after P35, which is the age of sexual maturity of the mouse. After intraperitoneal injections (IP) of ketamine (100 mg/kg) and xylazine (10 mg/kg), the pupils of the mice were dilated with 1% tropicamide (Bausch + Lomb, Inc., Tampa, FL) followed by IP injection of 10% fluorescein sodium (100 mg/kg) (AK-FLUOR; Akorn, Decatur, IL) 1 to 2 minutes before imaging. Using a commercially available Micron IV retinal imaging system (Phoenix Research Laboratories, Pleasanton, CA), retinal FA images with 50° fields of views were obtained in one eye of each mouse.
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Retinal Vascular Development Phases
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Fluorescein sodium
Ip injection
Ketamine
Mouse
Pupils
Retinal
Retinal vascular
Sexual maturity
Tropicamide
Xylazine
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Variable analysis
independent variables
- Retinal vascular development phase (early, mid, late, mature)
- Retinal fluorescein angiography (FA) images
- Age of mice (P16 to P34 for early, mid, late phases; P47 for mature phase)
- Intraperitoneal (IP) injection of ketamine (100 mg/kg) and xylazine (10 mg/kg)
- Pupil dilation with 1% tropicamide
- IP injection of 10% fluorescein sodium (100 mg/kg) 1-2 minutes before imaging
- Retinal imaging using Micron IV retinal imaging system with 50° fields of view
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