Profiling Inflammatory microRNAs in Macrophages

To determine the expression of inflammatory microRNAs, macrophages from C57BL/6 WT mice were treated with PTEN siRNA or scrambled siRNA as above. RNA was then extracted from the cells using Qiagen RNeasy mini kit according to the manufacturer’s instructions. cDNA was synthesized using the RT2 microRNA First Strand Kit (Qiagen) and applied to PCR focused immunopathology microRNA array plates (Qiagen) as previously shown (10 (link)). Normalization and statistical analysis of miRNA expression was conducted using SABiosciences’ Online PCR Array Data Analysis Web Portal as previously described (10 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Sisti F., Wang S., Brandt S.L., Glosson-Byers N., Mayo L., Son Y.M., Sturgeon S., Filgueiras L., Jancar S., Wong H., Dela Cruz C.S., Andrews N., Alves-Filho J.C., Cunha F.Q, & Serezani C.H. (2018). Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Science signaling, 11(528), eaai9085.

Publication 2018

RNeasy mini kit RT2 microRNA First Strand Kit PCR focused immunopathology microRNA array plates

C57bl mice Cdna Inflammatory Macrophages Mirna Pten Sirna

Corresponding Organization : Vanderbilt University Medical Center

Other organizations : Universidade de São Paulo, Cincinnati Children's Hospital Medical Center, Yale University

Top 5 similar protocols

Variable analysis

Dependent Variables not detected.
Unable to provide accurate variables.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!