Protocol detail

Rapamycin Modulates STAT3 Signaling in I/R

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For global I/R protocol, three groups were used: (1) C57 (n = 4), (2) db/db (n = 4)-DMSO and db/db-Rapamycin (100 nM) (LC Laboratories, MA, USA) (n = 6). Isolated hearts were infused with DMSO (solvent for rapamycin) and rapamycin. The dose of rapamycin was chosen based on our previous studies on rapamycin-induced cardioprotection against I/R injury [9 (link), 29 (link)]. To investigate STAT3 signaling, four groups were used (n = 5): (1) WT-DMSO, (2) WT-Rapamycin, (3) STAT3-deficient-DMSO and (4) STAT3-deficient-Rapamycin. Also, we isolated primary adult cardiomyocytes from these groups of mice (n = 4). Protein was analyzed from the hearts following global I/R (n = 3). Experimental protocol is documented in Fig. 1.

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Das A., Salloum F.N., Filippone S.M., Durrant D.E., Rokosh G., Bolli R, & Kukreja R.C. (2015). Inhibition of mammalian target of rapamycin protects against reperfusion injury in diabetic heart through STAT3 signaling. Basic research in cardiology, 110(3), 31.

Publication 2015

DMSO

Adult Cardiomyocytes Dmso Hearts Injury Mice Protein Rapamycin Solvent Stat3

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Variable analysis

independent variables

Group 1: C57 (n = 4)
Group 2: db/db-DMSO (n = 4)
Group 3: db/db-Rapamycin (100 nM) (n = 6)
Group 4: WT-DMSO (n = 5)
Group 5: WT-Rapamycin (n = 5)
Group 6: STAT3-deficient-DMSO (n = 5)
Group 7: STAT3-deficient-Rapamycin (n = 5)

dependent variables

Isolated heart function following global I/R
STAT3 signaling
Protein expression in hearts following global I/R (n = 3)

control variables

DMSO (solvent for rapamycin)

controls

Positive control: C57 group
Negative control: db/db-DMSO group

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