Genomic DNA samples from the patient and his parents (100 ng) were amplified by using two sets of primers specific to FOXL2 (NM_023067.3). Forward primer 1: 5′-GAGCTTAGGAAAGCGAAAAAGCAC AGAGGG-3′, reverse primer1: 5′-GAAGACATGTTCGAGAAGGGCAACTACCG-3′, forward primer 2: 5′-GTTGAGGAAGCCAGACTGCAGGTACTTGGG-3′, and reverse primer 2: 5′-TCTCCAGAAGTTTGAGACTTGGCCGTAAGC-3′. PCR reaction and conditions were as follows: Promega (Madison, WI) GoTaq Hot Start kit with 1× Master Mix and 400 nM of each primer. PCR began with an initial cycle at 95°C for 3 minutes, followed by 30 cycles of 94°C for 30 seconds, 60°C for 30 second and 72°C for 1 minute, finishing with extension at 72°C for 5 minutes. Amplified PCR products were sequenced using the PCR primers as sequencing primers on an ABI (Carlsbad, CA) PRISM 3730xl at a commercial sequencing facility. High resolution copy number analysis was performed using Affymetrix (Santa Clara, CA) SNP 500K arrays as previously described [Haldeman-Englert et al., 2010 (link)].