A 5-mL venous blood sample was collected from each participant after at least 12
hours of fasting. Part of the sample (2 mL) was placed in a glass tube and used
to perform biochemical assays. Another part of the sample (3 mL) was collected
in tubes containing anticoagulants (4.80 g/L citric acid, 14.70 g/L glucose,
13.20 g/L trisodium citrate) and was utilized to extract DNA. Genotyping of the
XKR6 rs7014968 SNP was performed by Snapshot technology in
the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co Ltd, China.23 (link),26 (link)–38 (link, link, link, no link found, no link found, link, link, link, link, link, link, link) The sequences of the forward and backward primers used were
5′-TGGAACTAATCGTTGTTGCCAGTC-3′ and 5′-TCCCAGTGAAAAGCAAACCAGAA-3′, respectively.
Seven serum lipid traits (TC, TGs, HDL-C, LDL-C, ApoA1, ApoB, and ApoA1/ApoB)
were determined as described in our previous reports.23 (link),26 (link)–38 (link, link, link, no link found, no link found, link, link, link, link, link, link, link) All determinations were completed with an autoanalyzer (Type 7170A;
Hitachi Ltd, Tokyo, Japan) in the Clinical Science Experiment Center of The
First Affiliated Hospital, Guangxi Medical University.