Chromatin from BMM was analyzed using a modification of the ChIP
Millipore/Upstate protocol (MCPROTO407) as described(29 (link)) using Magna ChIP Protein A+G Beads
(16-663, Millipore). The following antibodies were used for ChIP: EZH2 (Active
Motif, 39902), H3K27me3 (Active Motif, 39155), C/EBPβ C-terminal (Santa
Cruz, sc-150) and C/EBPβ-LAP (CST, 3087). Aliquots for input and
non-specific IgG control samples were included with each experiment.
ChIP-qPCR primers are listed inTable 2 . Fold enrichment was calculated based on Ct
as 2(ΔCt), where ΔCt = (CtInput –
CtIp). The IgG ΔCt was subtracted from the specific Ab
ΔCt to generate ΔΔCt = (ΔCtspecific Ab– ΔCtIgG).
Millipore/Upstate protocol (MCPROTO407) as described(29 (link)) using Magna ChIP Protein A+G Beads
(16-663, Millipore). The following antibodies were used for ChIP: EZH2 (Active
Motif, 39902), H3K27me3 (Active Motif, 39155), C/EBPβ C-terminal (Santa
Cruz, sc-150) and C/EBPβ-LAP (CST, 3087). Aliquots for input and
non-specific IgG control samples were included with each experiment.
ChIP-qPCR primers are listed in
as 2(ΔCt), where ΔCt = (CtInput –
CtIp). The IgG ΔCt was subtracted from the specific Ab
ΔCt to generate ΔΔCt = (ΔCtspecific Ab– ΔCtIgG).
