Quantifying HIV-RNA and DNA Levels
Corresponding Organization : Centre National de la Recherche Scientifique
Other organizations : Institut Pasteur, Infectious Disease Models and Innovative Therapies, CEA Paris-Saclay - Etablissement de Fontenay-aux-roses, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Assistance Publique – Hôpitaux de Paris, Université Paris-Sud, Institut Paoli-Calmettes, Université Paris Cité, Délégation Paris 5, University of Genoa, Istituti di Ricovero e Cura a Carattere Scientifico, Ospedale Policlinico San Martino, Sorbonne Université, Hôpital Tenon, Centre de recherche en Epidémiologie et Santé des Populations, Université Paris-Saclay
Variable analysis
- Quantification of HIV-RNA in plasma by real-time RT-PCR with the Cobas TaqMan HIV1 v2.0 assay (Roche Diagnostics)
- Quantification of total HIV-DNA in PBMC by ultra-sensitive real-time PCR using the Generic HIV-DNA assay from BioCentric (Bandol, France)
- Threshold values were arbitrarily given to samples below the threshold of the assay (20 RNA copies/mL)
- No positive or negative controls were explicitly mentioned in the input.
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