Enzymatic Activity Assay of rSeCP
Corresponding Organization :
Other organizations : Zhengzhou University
Protocol cited in 3 other protocols
Variable analysis
- Concentrations of Cu++, Mn++, and Zn++ metal ions (0.01 mM, 0.1 mM, 1 mM, 10 mM, and 100 mM)
- Inhibitors (PMSF, AEBSF, EDTA, E-64)
- Enzymic activity of rSeCP
- Fluorescence intensity
- Standard assay buffer (100 mM sodium acetate, 5 mM dithiothreitol (DTT); pH 5.5)
- Substrate concentration (3 μM)
- Reaction volume (240 μl)
- Incubation time (30 min)
- Incubation temperature (37°C)
- Excitation and emission wavelengths (355 nm and 460 nm)
- Buffers used for pH activity profiles (100 mM sodium acetate, 100 mM sodium phosphate, 100 mM Tris–HCl)
- Highest enzyme activity (100% relative activity)
- Control without reagents
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