Immunofluorescent analyses to evaluate macrophage
heterogeneity11 (link) were
completed with images of colabeled rabbit anti-IBA-1 (1:1000, Wako,
019–19741), rat anti-CD86 (1:200, BD, 553689), and goat anti-CD206
(1:200, R&D, AF2535), using AlexaFluor (AF) secondary antibodies donkey
anti-rabbit (1:200, AF647, Thermo Scientific, A31573), donkey anti-rat (1:200,
AF488, Thermo Scientific, A21208), and donkey anti-goat (1:200, AF546, Thermo
Scientific, A11056). Slides were coverslipped using mounting media with DAPI
counterstain (Vector Hardset Antifade Mounting Media), and stored at
−20°C until imaging. All imaging was performed with a Zeiss Axio
Scan.Z1 at magnification ×20.
heterogeneity11 (link) were
completed with images of colabeled rabbit anti-IBA-1 (1:1000, Wako,
019–19741), rat anti-CD86 (1:200, BD, 553689), and goat anti-CD206
(1:200, R&D, AF2535), using AlexaFluor (AF) secondary antibodies donkey
anti-rabbit (1:200, AF647, Thermo Scientific, A31573), donkey anti-rat (1:200,
AF488, Thermo Scientific, A21208), and donkey anti-goat (1:200, AF546, Thermo
Scientific, A11056). Slides were coverslipped using mounting media with DAPI
counterstain (Vector Hardset Antifade Mounting Media), and stored at
−20°C until imaging. All imaging was performed with a Zeiss Axio
Scan.Z1 at magnification ×20.
