Quantification of NG2 Protein Levels

Levels of NG2 in HBVP lysate were analysed using an in house developed assay based on Meso Scale Discovery electrochemiluminescence technology and their immunoassay conversion kits (Meso Scale Discovery, Rockville, MD). The procedure has been described previously.^{13 (link),19 (link)} The electrochemiluminescence signal was quantified using a Meso Scale Discovery SECTOR Imager 6000. The NG2 levels were analysed in lysates obtained from three separate experiments with three replicates for each experiment. Protein concentration in cell lysates was analysed using a BCA Protein Assay Kit according to manufacturer’s instructions. Analysis of protein concentrations showed no differences between the stimulated wells (data not shown).