Male CD1 nude mice (Charles River, Milan, Italy) were maintained under the guidelines established by our Institution (University of L’Aquila, Medical School and Science and Technology School Board Regulations, complying with the Italian government regulation n.116 27 January 1992 for the use of laboratory animals). All mice were anesthetized with a mixture of ketamine (100 mg/kg)/xylazine (5 mg/kg) in saline and subsequently received S.C. flank injections of 1 × 106 PC3 and 22v1 cells and DTX resistant strains. Tumour growth was assessed by bi-weekly measurement of tumour diameters with a Vernier calliper (length × width). Randomization was performed when subcutaneous tumors reached volumes ranged between 80 and 100 mm3. This was commonly obtained 7–10 days after cell injection. Tumour weight was calculated according to the formula: TW (mg) = tumour volume (mm3) = d2 × D/2, where d and D are the shortest and longest diameters, respectively. The effects of the treatments were examined as previously described [50 (link)].
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