All chemicals and reagents were purchased from Sigma Aldrich (St. Louis, Michigan, USA) unless otherwise stated. For sample homogenization, two types of micropestles were acquired, one from Sigma Aldrich (#BAF199230001) and one Optima Inc., Glencoe, IL, USA, (#320302). Filter-aided sample preparation (FASP) [37 (link),38 (link)] was conducted on Microcon® Centrifugal Filters (30 kDa molecular cut-off, Merck KGaA #MRCF0R030, purchased through Sigma Aldrich. For bead-based sample preparation, ferromagnetic beads with MagReSyn® Amine functional groups (ReSyn Biosciences, Gauteng, South Africa) were used. For sample preparation using suspension trapping, S-Trap [51 (link)] micro-cartridges with a binding capacity of <100 µg total protein were purchased from ProtiFi (Farmingdale, NY, USA, #CO2-micro-80).
The total protein concentration was determined using a reducing-agent-compatible Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA, #23250) following the manufacturer’s instructions.
The total protein concentration was determined using a reducing-agent-compatible Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA, #23250) following the manufacturer’s instructions.
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