Fresh C. asiatica aerial parts was obtained locally and processed as previously reported.26 (link) The plant sample was washed with tap water and rinsed with reverse osmosis water. The aerial parts were air-dried to reduce moisture content for 12 hour and further oven dried at 60 °C for another 12 hours. The dried sample was ground into fine powder and stored in an opaque bottle at −20 °C for further extraction.
Dried C. asiatica powder (50 g) was extracted using 500 mL of ethanol/water mixture (80 : 20 v/v) for 2 hours while gently stirring with an overhead stirrer. The mixture was filtered using a Buchner funnel overlaid with Whatman No 1 filter paper. The collected marc was re-extracted. The combined filtrate was concentrated using rotary evaporator at 40 °C to one-third of its original volume. Thereafter, the filtrate was kept at 4 °C overnight. A clear supernatant was obtained and subjected to reduced pressure in a speed-vac in order to remove any residual organic solvent. The mixture was filtered using Whatman No. 1 filter paper under gravity and then lyophilized to give a light brown powder (Fig. 1 ) hereafter referred to as C. asiatica phenolic extract (CAPE). CAPE was stored at −20 °C protected from light.
Dried C. asiatica powder (50 g) was extracted using 500 mL of ethanol/water mixture (80 : 20 v/v) for 2 hours while gently stirring with an overhead stirrer. The mixture was filtered using a Buchner funnel overlaid with Whatman No 1 filter paper. The collected marc was re-extracted. The combined filtrate was concentrated using rotary evaporator at 40 °C to one-third of its original volume. Thereafter, the filtrate was kept at 4 °C overnight. A clear supernatant was obtained and subjected to reduced pressure in a speed-vac in order to remove any residual organic solvent. The mixture was filtered using Whatman No. 1 filter paper under gravity and then lyophilized to give a light brown powder (
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