Dual Immunofluorescence Detection of PGC-1α and NeuN

Double immunofluorescence staining [5 (link),7 (link),8 (link),43 (link),56 (link)] was carried out using a goat polyclonal antiserum against PGC-1α and NRF1 (Santa Cruz Biotechnology) and a mouse monoclonal antiserum against a specific neuronal marker, neuron-specific nuclear protein (NeuN; Chemicon). The secondary antisera included goat anti-rabbit IgG conjugated with AlexaFluor 488 and goat anti-mouse IgG conjugated with Alexa Fluor 568 (Molecular Probes, Eugene, OR, USA). Sections were viewed under an Olympus AX-51 epifluorescence microscope (Olympus, Kyoto, Japan); immunoreactivity for NeuN exhibited red fluorescence and PGC-1α manifested green fluorescence.

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Chuang Y.C., Chen S.D., Hsu C.Y., Chen S.F., Chen N.C, & Jou S.B. (2019). Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway. International Journal of Molecular Sciences, 20(4), 998.

Publication 2019

goat polyclonal antiserum against PGC-1α and NRF1 NeuN goat anti-rabbit IgG conjugated with AlexaFluor 488 goat anti-mouse IgG conjugated with Alexa Fluor 568 AX-51 epifluorescence microscope

Alexa 568 Anti igg Antisera Fluorescence Goat Immunofluorescence Microscope Molecular probes Mouse Neuron Nuclear protein Pgc 1α Rabbit

Corresponding Organization : Mackay Medical College

Other organizations : Kaohsiung Chang Gung Memorial Hospital, Chang Gung University, National Sun Yat-sen University, Institute for Biomedicine, Kaohsiung Medical University

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Variable analysis

independent variables

Goat polyclonal antiserum against PGC-1α and NRF1
Mouse monoclonal antiserum against neuron-specific nuclear protein (NeuN)

dependent variables

Immunoreactivity for NeuN (red fluorescence)
Immunoreactivity for PGC-1α (green fluorescence)

control variables

Secondary antisera: Goat anti-rabbit IgG conjugated with AlexaFluor 488 and goat anti-mouse IgG conjugated with Alexa Fluor 568

controls

Positive control: None explicitly mentioned
Negative control: None explicitly mentioned

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