Lymphocyte Surface Marker Analysis

Lymphocytes were isolated from spleen, as described above. According to the method described in our previous studies [3 (link), 40 (link)], surface staining was performed by incubation with surface markers, including phycoerythrin-(PE)-labeled anti-mouse CD3, allophycocyanin (APC)-labeled anti-mouse CD4, and fluorescein isothiocyanate (FITC)-labeled anti-mouse CD8 (eBioscience). The cell suspension was then fixed with FACScan buffer (PBS containing 1% BSA and 0.1% sodium azide) and 2% paraformaldehyde. All data were acquired through a FACScan flow cytometer (BD Biosciences, USA). The analysis was performed with the data from three independent experiments.

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Zhu Y.C., He Y., Liu J.F, & Chen J. (2020). Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice. Parasite, 27, 26.

Publication 2020

PE)-labeled anti-mouse CD3 allophycocyanin (APC)-labeled anti-mouse CD4 FITC)-labeled anti-mouse CD8 FACScan

Allophycocyanin Anti cd3 Buffer Cell Fluorescein Isothiocyanate Lymphocytes Mouse Paraformaldehyde Phycoerythrin Sodium azide Spleen

Corresponding Organization :

Other organizations : Ningbo University

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Variable analysis

independent variables

Surface markers, including phycoerythrin-(PE)-labeled anti-mouse CD3, allophycocyanin (APC)-labeled anti-mouse CD4, and fluorescein isothiocyanate (FITC)-labeled anti-mouse CD8

dependent variables

Cell surface expression of CD3, CD4, and CD8 markers

control variables

Cell suspension fixed with FACScan buffer (PBS containing 1% BSA and 0.1% sodium azide) and 2% paraformaldehyde
Data acquired through a FACScan flow cytometer (BD Biosciences, USA)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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