Detailed SDS-PAGE and Western Blot Protocol

Cell lysates were obtained as described previously (Sluysmans et al., 2021 (link)) with the following modifications: SDS-PAGE was carried out at RT, and transfer onto nitrocellulose membrane was carried out at 85 V for 120 min. Blocking was realized with Tris-buffered saline (TBS) with 0.1% Tween-20 and 5% low-fat milk, and incubation with primary and secondary antibodies in the same buffer containing only 3% low-fat milk.
Chemiluminescence (ECL) was detected using Odyssey Imager (LI-COR) or Amersham ImageQuant 800 (Cytiva). Numbers on the left of immunoblots correspond to sizes in kilodaltons (kDa) of prestained markers. Uncropped blots are shown in Fig. S8.