Measurement of serum TG was performed using the Wako LabAssay Triglyceride kit (290-63701) (Wako Chemicals USA). Liver TG was determined through the measurement of its glycerol contents38 (link). Briefly, liver tissue chunks were subjected to saponification in ethanolic KOH, and the released free glycerol from TG was measured with the Free Glycerol Reagent (F6428, Sigma-Aldrich).
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