Quantitative Immunoblotting: Validated Protocols

Immunoblotting assays were performed, as previously described and validated by our group described [36 (link),37 (link),38 (link),39 (link),40 (link)] and developed with the Odyssey system (LI-COR Biosciences, Lincoln, NE, USA). The intensity of the bands was quantified by using the FIJI (Fiji Is Just ImageJ) software. Antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): CREB (catalog number: #4820), Phospho-CREB (pCREB Ser¹³³; catalog number: #9198), ERK1/2 (catalog number: #9102); and from Santa Cruz Biotechnology (Dallas, TX, USA): p-ERK Antibody (catalog number: #sc-7383).

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Matarese A., Gambardella J., Lombardi A., Wang X, & Santulli G. (2020). miR-7 Regulates GLP-1-Mediated Insulin Release by Targeting β-Arrestin 1. Cells, 9(7), 1621.

Publication 2020

Odyssey system ERK1/2 p-ERK Antibody

Antibodies Antibody Erk1 Immunoblotting assays

Corresponding Organization : Albert Einstein College of Medicine

Other organizations : Ospedale Antonio Cardarelli

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Intensity of the bands quantified by using the FIJI (Fiji Is Just ImageJ) software

control variables

None explicitly mentioned

controls

The immunoblotting assays were performed as previously described and validated by the authors' group in the referenced publications [36, 37, 38, 39, 40].

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