Isolation and Culture of Rat Cardiac Stem Cells

We obtained rat CSCs from the hearts of WKY rats as described in previously published papers^{12 (link),26 (link),27 (link)}. Briefly, hearts were minced into fragments less than 2 mm³, followed by digestion with collagenase and then seeded onto fibronectin-coated plates to create explant-derived cells. In about 14 days, explant-derived cells were harvested and seeded in ultralow attachment flasks (Corning Life Sciences, Durham, NC) and grown in suspended culture for cardiosphere formation. CSCs were obtained by replating cardiospheres on fibronectin-coated plates.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Tang J., Su T., Huang K., Dinh P.U., Wang Z., Vandergriff A., Hensley M.T., Cores J., Allen T., Li T., Sproul E., Mihalko E., Lobo L.J., Ruterbories L., Lynch A., Brown A., Caranasos T.G., Shen D., Stouffer G.A., Gu Z., Zhang J, & Cheng K. (2018). Targeted repair of heart injury by stem cells fused with platelet nanovesicles. Nature biomedical engineering, 2, 17-26.

Publication 2018

ultralow attachment flasks

Cells Collagenase Digestion Fibronectin Hearts Wky rats

Corresponding Organization :

Other organizations : North Carolina State University, Soochow University, Nagasaki University, University of North Carolina at Chapel Hill, First Affiliated Hospital of Zhengzhou University

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

Digestion of heart fragments with collagenase
Seeding of explant-derived cells onto fibronectin-coated plates
Culture of explant-derived cells in ultralow attachment flasks for cardiosphere formation
Replating of cardiospheres onto fibronectin-coated plates to obtain CSCs

dependent variables

Cardiosphere formation
Successful isolation of CSCs

control variables

Heart fragment size (less than 2 mm^3)
Rat strain (WKY rats)
Fibronectin-coated plates for cell culture

positive controls

Previous studies that describe the isolation of rat CSCs (references 12, 26, and 27)

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!