Total RNA isolated from ECFCs of two healthy adult individuals (every 2 samples, N = 4) and the IP (2 samples) were subjected to the high-throughput RNA-seq. After quantity and quality evaluations, RNA-seq libraries were generated using QuantSeq 3’-mRNA Library Prep kit (Lexogen, Wien, Austria) and subsequently sequenced on an Illumina HiSeq 2500 V4 platform (Illumina, San Diego, CA, USA). After quality assessment and trimming tasks, the obtained reads were aligned to the reference human genome GrCH38.8 (Ensemble) using the miARMa pipeline [60 (link),61 (link),62 (link)]. Tophat2 and bowtie2 were used to complete mapping [61 (link),63 (link)]. The reads were converted to FPKM (fragments per kilobase of transcript per million mapped reads), and the additional quality control was performed via Qlucore Omics Explorer 3.6 (Qlucore, Lund, Sweden). The genes with p-value ≤ 0.05 and fold change ≥2 (or a mean difference (absolute log2FC) > 1) were considered statistically significant DEGs.
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