The detailed experiment is well explained in our previous study [17 (link)]. Briefly, the neurotracer dye, Fluoro-gold (5% solution in saline, Invitrogen, NY, USA) was injected to the superior colliculus of mice using a piece of soaked Gelfoam. The eyes were taken out seven days after the Fluoro-gold application, and the retinas were detached at the ora serrata, followed by cutting with a trephine near the optic nerve head. After four radial incisions, the retinal flat mounts were prepared on silane-coated microscope slides.
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