Regulation of PPARα and Caspase-3 in PANC-1 Cells

PANC-1 cells were treated separately with DMSO (control), 60 μg/mL EGCG, 20 μM Y-27632, and 60 μg/mL EGCG + 20 μM Y-27632 for 48 h. Quantitative real-time polymerase chain reaction (RT-qPCR) [19 (link)] was used to observe the expression of PPARα mRNA and caspase-3 mRNA of these groups. The primer pairs [glyceraldehyde-3-phosphate dehydrogenase (GAPDH), PPARα, and caspase-3] were synthesized by Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China). The primer pairs included the following: forward: 5′-AGAAGGCTGGGGCTCATTTG-3′ and reverse: 5′-AGGGGCCATCCACAGTCTTC-3′ for GAPDH (258 bp); forward: 5′-TTCGCAATCCATCGGCGAG-3′ and reverse: 5′-CCACAGGATAAGTCACCGAGG-3′ for PPARα (146 bp). Forward: 5′-CATGGAAGCGAATCAATGGACT-3′ and reverse: 5′-CTGTACCAGACCGAGATGTCA-3′ for caspase-3 (139 bp). GAPDH was used as an internal control to evaluate the relative expression of PPARα. RT-qPCR reagents were purchased from TIANGEN Biotech (Beijing) Co., Ltd. (Beijing, China). Relative mRNA was calculated using the formula: 2−ΔΔCt [20 (link),21 (link)].