In line with a previous protocol,
18 (link) IHC staining was performed using immunohistochemical staining kit (zsbio; Cat No. SP‐9000). The primary and secondary antibodies are listed in TableS1 . The sections were analyzed simultaneously by two independent investigators. Cell staining was evaluated following the immunoreactive score (IRS) proposed by Remmele and Stegner with slight modifications.
20 (link) For statistical analyses, cases with an IRS of 0–3 were defined as negative; otherwise, they were defined as positive. The cutoff scores for low and high ILT4 expression were <4 and ≥4, respectively. Microvessel density (MVD) was calculated according to a modification of Weidner's method.
21
18 (link) IHC staining was performed using immunohistochemical staining kit (zsbio; Cat No. SP‐9000). The primary and secondary antibodies are listed in Table
20 (link) For statistical analyses, cases with an IRS of 0–3 were defined as negative; otherwise, they were defined as positive. The cutoff scores for low and high ILT4 expression were <4 and ≥4, respectively. Microvessel density (MVD) was calculated according to a modification of Weidner's method.
21
